Detection and Characterization of Candida albicans Isolated from Clinical Specimens
DOI:
https://doi.org/10.59675/Keywords:
Detection, Characterization, Candida albicans isolated, clinical, specimensAbstract
Candida albicans are reportedly the most common human fungal pathogens. The objective was to analyse the distribution of C. albcans among isolates from various clinical specimens and to ascertain how their virulence factors were expressed. Methods: C. albicans isolates were collected from clinical specimens at Karbala hospitals between March 2022 and November 2022, a descriptive study on the makeup of Candida specimens’ identification by phenotype and genotype test. One hundred clinical specimens, 35 oral swabs (patient Diabetes), 35 oral swabs (patient non-diabetes) and 30 throat swabs, were obtained from various locations and grown on chromogenic media. The total (n=60) isolates were identified as C. albicans using biochemical and partial sequence of 18S rDNA sequences analysis. All C. albicans isolates named CA1 to CA 60 were characterised by drug resistance (CDR1) gene and multidrug resistance (MDR1) gene. Results: C. albicans was the most prevalent (60). Our findings support the quick and precise identification of C. Albcans infections in clinical specimens using the 18S rDNA PCR diagnostic workflow. C. albicans isolates showed 55 % and 40 % (CDR1 and MDR1) genes, respectively. The PCR results reported here suggest that amplification with CDR1 and MDR1 genes may be helpful for specifically identifying C. albicans strains.
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